Due to its higher sensitivity and its reliability, RNA synthesis assay using HepG2 cell line provides the most suitable tool for the screening of water contaminants. ATP and AB assays responded to a similar pattern. Except with 4-OP, RNA assay was always inhibited at lower concentrations than those required in the other assays, suggesting that this assay is a very sensitive indicator of the presence of toxic compounds. Statistical analysis revealed a correlation between the IC50 values obtained in the three assays. Furthermore, BP was not cytotoxic in the ATP and AB assays. Among all substances, we were not able to determine atrazine IC50 value whatever the test used. Using Graphpad Prism software, an XY table is created, with the logarithm of the concentration of the. The equation used to calculate the viability percentage is: V i a b i l i t y () A v e r a g e a b s o r b a n c e c o n d i t i o n A v e r a g e a b s o r b a n c e c o n t r o l × 100. The in vitro toxicological index IC50 (the millimolar concentration of compound which inhibits response assay by 50% compared to the solvent control) of 11 water contaminants (acrylamide, atrazine, BP, BPA, 2,4-DAT, 17-αEE, H 2O 2, 4-OP, sodium bromate, sodium chlorate, sodium nitrate) was evaluated on the human hepatoma (HepG2) cells using three short-term bioassays related to their morbidity status. The control condition is set as 100 viability.

Ic50 graphpad prism percent viability